NGS Workflow Steps - Next-Generation Sequencing

Unlocking The Most Cost-Effective & Reproducible NGS Workflow

Although reaction miniaturization is widely adopted in large pharmaceutical settings, it can still be difficult to navigate the challenges associated with producing high-quality data in this context.  

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The DISPENDIX team has successfully tackled miniaturization challenges in even the most intricate NGS workflows. Our expertise allows you to scale volumes down to 1/10th of the manufacturer's recommended standard operating procedures (SOP), all while maintaining exceptional data quality, as many in the industry have benefitted from.

Normalization & Pooling

Once the library has been prepared, conducting quality control analysis reveals the concentration of each individual NGS library. When samples are pooled for economical sequencing, it's essential to normalize them to the same concentration to prevent oversampling of any one library over others.

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The I.DOT system can normalize up to 96 NGS libraries within minutes while maintaining a mere 1 μL dead volume per sample. This presents a significantly more efficient alternative to manual pipetting and conventional automated liquid handlers, which is a game changer for many labs.

Moreover, the I.DOT can pool variable volumes of each sample into a single well, allowing for normalization and pooling to occur seamlessly in one step—now that's true efficiency!

Are you curious about our NGS Library Prep Kits? Reach out to us today to arrange for an expert consultation!